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KMID : 0350519940470041705
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1705 ~ p.1715
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The Effect of L-Carnosine and Thrombin on DNA and Collagen Synthesis of Cultured Human Skin Fibroblasts
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Abstract
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It has been well known that the proliferation of fibroblasts plays a crucial role in wound healing process and thrombin has the mitogenic activity. It has been reported that L-carnosine exerted a positive and promotive effect on the wound healing
process and increased the tensile strength and contents of hydroxyproline in healing tissue of dermal wound. However, there are no reports on the effect of L-carnosine in the DNA and collagen synthesis of the cultured human skin fibroblasts.
Therefore,
in this report, we examined the effect of L-carnosine(β-alanyl-L-histidine) and thrombin on the DNA and collagen synthesis, and expression of mRNA for collagen were studied in cultured human fibroblasts.
The incorporation rates of [3H]-thymidine and [3H]-proline were measured at 2 or 5 hours after the administration of β-alanine, L-histidine, L-carnosine and thrombin into the cultured human skin fibroblasts.
@ES The results were as follows:
@EN 1) The optimal doses of I-carnosine and thrombin were 1×10E-2 and 1 unit, respectively, in the incorporation rates of[3H]-thymidine into the cultured human skin fibroblasts(P<0.001).
2) The administration of L-carnosine or thrombin profound dly increased the DNA synthesis of the cultured human skin fibroblasts, with the significant increase of the DNA synthesis in β-alanine-or L-histidine-treated group(P<0.001).
3) The collagen synthesis of the cultured human skin fibroblasts was significantly increased at 2 hours after the treatment of β-alanine, L-histidine or thrombin with the maximum increase in L-carnosine-treated group. The collagen synthesis of
the
cultured human skin fibroblasts was increased at 5 hours after the treatment of L-histidine, L-carnosine or thrombin with the maximum increase in L-carnosine-treated group.
4) The expression of mRNA for Type I collagen a-2 chain of the cultured human skin fibroblasts was enhanced at 2 hours after the treatment of L-carnosine. However, no enhancement was found in β-alanine, L-histidine- or thrombin-treated group.
The results demonstrated that the L-carnosine accelerated both DNA and collagen synthesis, however, the thrombin enhanced DNA synthesis, but no collagen sythesis of cultured human skin fibroblasts.
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